AnswerB only which I marked
Sample 4: Primary antibody: W6/32 (MHC class 1) Secondary antibody: rabbit anti-mouse FITC Condition: 4°C Please note: this image shows six cells at a smal scale than sample 3
Sample 3: Primary antibody: W6/32 (MHC class 1) Secondary antibody: rabbit anti-mouse FITC Condition: 22°C Please note: this image shows one single cell that has been enlarged
Table 1 Experimental conditions and antibodies. Experimental conditions Primary antibody staining Secondary antibody staining Secondary Secondary antibody antibody type volume (PL) Specificity Incubation temperature (°C) Incubation time (min) Primary antibody name Primary antibody volume (PL) Tube 10 22 20 10 Rabbit anti- mouse Isotype control Rabbit anti- mouse FITC Rabbit Tube 10 Rabbit anti- mouse Isotype control anti- mouse FITC Rabbit Tube 22 20 W6/32 MHC class 10 anti- Tube W6/32 MHC class 10 Tube HB55 MHC class 10 mouse FITC Rabbit anti- mouse FITC Rabbit anti- mouse FITC Rabbit anti- mouse FITC Tube HB55 MHC class 10 Materials: Cells: THP1 – human myelomonocytic continuous cell line derived from a leukaemia patient Primary antibodies: Rabbit anti-mouse (unlabelled): Produced in rabbit, specific for mouse antigens only. W6/32 (unlabelled): Raised in mouse, specific for human MHC class 1 receptor, expressed in all somatic cells (98% of cells) HB55 (unlabelled): Raised in mouse, specific for human MHC class II receptor, expressed only in cells involved in antigen presentation such as macrophages, B cells, etc. Secondary antibodies: Rabbit anti-mouse- FITC: Raised in rabbit, specific to mouse antigens and labelled with FITC fluorophore for fluorescent visualisation under microscope (diluted 1:10 from stock! preparation). (FITC is sensitive to light; therefore secondary antibody is wrapped in the silver alls that is no chlorof low
ASSESSMENT OF PART 1 – IMMUNOLOGICAL RECEPTOR EXPRESSIUN a) Draw and label the fluorescence staining patterns of the cells at 4 °C and at 22 °C. [5 marks] b) Describe the staining patterns seen and briefly explain the biological basis of patching’ and ‘capping’ of receptors on the cell surface of lymphocytes, as seen in the data. (10 marks] c) Write an abstract summarising this experiment (approx. 200 words) in the style of a scientific paper format (SPF). [25 marks]
The post Draw and label the fluorescence staining patterns of the cells at 4 °C and at 22 °C appeared first on nursing assignment tutor.